Biological activity of carnitine 2-ethyl-6-methyl-3-hydroxypyridine

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Abstract

The antiradical properties and biological activity of carnitine 2-ethyl-6-methyl-3-hydroxypyridine (СP) were investigated. The drug had high antiradical activity. In the concentration range 10-6–10-9M, CP prevented the activation of lipid peroxidation in the membranes of mouse liver mitochondria incubated in a hypotonic medium. Such an incubation caused a change in the fatty acid (FA) composition of the lipid component of mitochondrial membranes: the total relative percentage of 18:2ω6, 18:1ω9 and 22:6ω3 – the main FAs that make up cardiolipin decreased by 8.1%. The introduction of CP into the incubation medium led not only to the restoration of the pool of these FAs, but also to an increase in their content by 15%, which, possibly, contributed to an increase in the efficiency of mitochondrial functioning and an increase in the body’s resistance to stress.

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About the authors

I. V. Zhigacheva

Emanuel Institute of Biochemical Physics, Russian Academy of Sciences

Author for correspondence.
Email: zhigacheva@mail.ru
Russian Federation, Moscow

I. F. Rusina

Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences

Email: zhigacheva@mail.ru
Russian Federation, Moscow

N. I. Krikunova

Emanuel Institute of Biochemical Physics, Russian Academy of Sciences

Email: zhigacheva@mail.ru
Russian Federation, Moscow

T. L. Veprintsev

Emanuel Institute of Biochemical Physics, Russian Academy of Sciences

Email: zhigacheva@mail.ru
Russian Federation, Moscow

Y. V. Kuznetsov

Emanuel Institute of Biochemical Physics, Russian Academy of Sciences

Email: zhigacheva@mail.ru
Russian Federation, Moscow

M. M. Rasulov

State Research Institute of Chemistry and Technology of Organoelement Compounds

Email: zhigacheva@mail.ru
Russian Federation, Moscow

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2. 1. Kinetic curve of chemiluminescence quenching accompanying the initiated oxidation of ethylbenzene with 2-ethyl-6-methyl-3-hydroxypyridine carnitinate at [KP]0 = 5.2·10-6M; Wi =1.12.10-8 M·c-1; activator – [DBA] =5.10-3M; 50 C; t0.5 is the period of inhibition of oxidation.

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3. 2. The effect of “aging” and various concentrations of CP on the fluorescence intensity of lipid peroxidation products in the membranes of mouse liver mitochondria. On the ordinate axis is the fluorescence intensity in units/mg of protein; on the abscissa axis is the concentration of KP; 1 is “aging” + KP; 2 – control + KP.

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4. Fig. 3. C18 LC unsaturation index: 1 – control; 2 – incubation in a hypotonic environment; 3 – incubation in a hypotonic environment with an injection of 10-9 M KP; 4 – incubation in a hypotonic environment with an injection of 10-6 M KP.

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