Mechanism of Action of NvZhen ErXian HeJi in Ovariectomized Rats with Myocardial Infarction based on Network Pharmacology


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Abstract

Objective:NvZhen ErXian HeJi (NZEXHJ) is used to treat perimenopausal syndrome (PS), but its effect on perimenopausal coronary heart disease is unclear. Furthermore, the aim of this research is to study the effect of NZEXHJ on perimenopausal coronary heart disease (PMCHD) in a rat model based on a network pharmacology approach.

Materials and Methods:Based on network pharmacological analysis combined with molecular docking, we predicted the potential therapeutic target and pharmacological mechanism of NZEXHJ in the treatment of PMCHD. We used an ovariectomized rat (OVR) model to understand the effect of NZEXHJ on myocardial injury and further verified the target of NZEXHJ in the intervention of PMCHD.

Results:We selected 52 active components of NZEXHJ against PMCHD and an intersection of their targets on network pharmacology, to which SCN5A, SER1, AR, and PGR were significantly correlated. The protein- protein interaction network revealed CASP3, CXCL8, IL6, MAPK1, TNF, TP53, and VEGFA in the treatment of PMCHD with NZEXHJ. Kaempferol, luteolin, and mistletoe presented good affinity towards the aforementioned targets by Molecular docking NZEXHJ exerted protecting cardiomyocytes for OVR. The mechanism was related to a reduction in the expression levels of the CXCL8, TNF, and regulating PI3K-AKT signaling pathways.

Conclusion:This study reveals the potential multi-component, multi-target, and multi-pathway pharmacological effects of NZEXHJ and predicts its protection against myocardial infarction in ovariectomized rats through the PI3K Akt pathway, providing a theoretical basis for the treatment of PMCHD.

About the authors

Kai Wu

Department of Cardiology, The First Affiliated Hospital of Xinxiang Medical University

Email: info@benthamscience.net

Shuxun Guo

Department of Cardiology,, The First Affiliated Hospital of Xinxiang Medical University

Email: info@benthamscience.net

Jie Zhang

Department of Cardiology,, The First Affiliated Hospital of Xinxiang Medical University

Email: info@benthamscience.net

Desong Wen

Department of Cardiology,, The First Affiliated Hospital of Xinxiang Medical University

Email: info@benthamscience.net

Linli Zhang

Department of Cardiology,, The First Affiliated Hospital of Xinxiang Medical University

Email: info@benthamscience.net

Mingyang Zhu

Department of Cardiology,, The First Affiliated Hospital of Xinxiang Medical University

Email: info@benthamscience.net

Xiulong Wang

Department of Cardiology,, The First Affiliated Hospital of Xinxiang Medical University

Email: info@benthamscience.net

Xuefang Li

Department of Cardiology, The First Affiliated Hospital of Xinxiang Medical University

Email: info@benthamscience.net

Zhigang Chen

Department of Cardiology, The First Affiliated Hospital of Xinxiang Medical University

Author for correspondence.
Email: info@benthamscience.net

Fei Lin

Department of Cardiology,, The First Affiliated Hospital of Xinxiang Medical University

Author for correspondence.
Email: info@benthamscience.net

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