Huangqi Guizhi Wuwu Decoction Improves Inflammatory Factor Levels in Chemotherapy-induced Peripheral Neuropathy by Regulating the Arachidonic Acid Metabolic Pathway


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Abstract

Background:Chemotherapy-induced Peripheral Neuropathy (CIPN) is a common complication that arises from the use of anticancer drugs. Huangqi Guizhi Wuwu Decoction (HGWWD) is an effective classic prescription for treating CIPN; however, the mechanism of the activity is not entirely understood.

Objective:This study aimed to investigate the remedial effects and mechanisms of HGWWD on CIPN.

Methods:Changes in behavioral, biochemical, histopathological, and biomarker indices were used to evaluate the efficacy of HGWWD treatment. Ultra-high-performance liquid chromatography/mass spectrometry combined with the pattern recognition method was used to screen biomarkers and metabolic pathways related to CIPN. The results of pathway analyses were verified by protein blotting experiments.

Results:A total of 29 potential biomarkers were identified and 13 metabolic pathways were found to be involved in CIPN. In addition HGWWD reversed the levels of 19 biomarkers. Prostaglandin H2 and 17α,21-dihydroxypregnenolone were targeted as core biomarkers.

Conclusion:This study provides scientific evidence to support the finding that HGWWD mainly inhibits the inflammatory response during CIPN by regulating arachidonic acid metabolism.

About the authors

Shanshan Wang

State Key Laboratory of Integration and Innovation of Classical Formula and Modern Chinese Medicine, National TCM Key Laboratory of Serum Pharmacochemistry, Metabolomics Laboratory, Department of Pharmaceutical Analysis, National Chinmedomics Research Center, Heilongjiang University of Chinese Medicine

Email: info@benthamscience.net

Xiaohui Du

State Key Laboratory of Integration and Innovation of Classical Formula and Modern Chinese Medicine, National TCM Key Laboratory of Serum Pharmacochemistry, Metabolomics Laboratory, Department of Pharmaceutical Analysis, National Chinmedomics Research Center,, Heilongjiang University of Chinese Medicine

Email: info@benthamscience.net

Guangli Yan

State Key Laboratory of Integration and Innovation of Classical Formula and Modern Chinese Medicine, National TCM Key Laboratory of Serum Pharmacochemistry, Metabolomics Laboratory, Department of Pharmaceutical Analysis, National Chinmedomics Research Center,, Heilongjiang University of Chinese Medicine

Email: info@benthamscience.net

Le Yang

State Key Laboratory of Dampness Syndrome,, The Second Affiliated Hospital Guangzhou Medical University

Email: info@benthamscience.net

Hui Sun

State Key Laboratory of Integration and Innovation of Classical Formula and Modern Chinese Medicine, National TCM Key Laboratory of Serum Pharmacochemistry, Metabolomics Laboratory, Department of Pharmaceutical Analysis, National Chinmedomics Research Center, Heilongjiang University of Chinese Medicine

Email: info@benthamscience.net

Xiwu Zhang

State Key Laboratory of Integration and Innovation of Classical Formula and Modern Chinese Medicine, National TCM Key Laboratory of Serum Pharmacochemistry, Metabolomics Laboratory, Department of Pharmaceutical Analysis, National Chinmedomics Research Center,, Heilongjiang University of Chinese Medicine

Email: info@benthamscience.net

Ling Kong

State Key Laboratory of Integration and Innovation of Classical Formula and Modern Chinese Medicine, National TCM Key Laboratory of Serum Pharmacochemistry, Metabolomics Laboratory, Department of Pharmaceutical Analysis, National Chinmedomics Research Center,, Heilongjiang University of Chinese Medicine

Email: info@benthamscience.net

Ying Han

State Key Laboratory of Integration and Innovation of Classical Formula and Modern Chinese Medicine, National TCM Key Laboratory of Serum Pharmacochemistry, Metabolomics Laboratory, Department of Pharmaceutical Analysis, National Chinmedomics Research Center, Heilongjiang University of Chinese Medicine

Email: info@benthamscience.net

Di Han

State Key Laboratory of Integration and Innovation of Classical Formula and Modern Chinese Medicine, National TCM Key Laboratory of Serum Pharmacochemistry, Metabolomics Laboratory, Department of Pharmaceutical Analysis, National Chinmedomics Research Center,, Heilongjiang University of Chinese Medicine

Email: info@benthamscience.net

Songyuan Tang

State Key Laboratory of Integration and Innovation of Classical Formula and Modern Chinese Medicine, National TCM Key Laboratory of Serum Pharmacochemistry, Metabolomics Laboratory, Department of Pharmaceutical Analysis, National Chinmedomics Research Center, Heilongjiang University of Chinese Medicine

Email: info@benthamscience.net

Xijun Wang

State Key Laboratory of Integration and Innovation of Classical Formula and Modern Chinese Medicine, National TCM Key Laboratory of Serum Pharmacochemistry, Metabolomics Laboratory, Department of Pharmaceutical Analysis, National Chinmedomics Research Center, Heilongjiang University of Chinese Medicine

Author for correspondence.
Email: info@benthamscience.net

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