Matrine-loaded Nano-liposome Induces Apoptosis in Human Esophageal-squamous Carcinoma KYSE-150 Cells


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Abstract

Introduction:Esophageal-Squamous Cell Carcinoma (ESCC) is often diagnosed at the middle or late stage, thus requiring more effective therapeutic strategies. Pharmacologically, the anti-tumor activity of the principal active constituent of Sophora flavescens, matrine (MA), has been explored widely. Notwithstanding, it is significant to nanotechnologically enhance the anti-tumor activity of MA in view of its potential to distribute non-tumor cells.

Methods:Herein, MA-loaded Nano-Liposomes (MNLs) were prepared to enhance the effect of anti-ESCC. The MNL showed a smaller sized particle (25.95 ± 1.02 nm) with a low polydispersed index (PDI = 0.130 ± 0.054), uniform spherical morphology, good solution stability, and encapsulated efficiency (65.55% ± 2.47). Furthermore, we determined the characteristics of KYSE-150 cells by cell viability assay, IC50, Mitochondrial Membrane Potential (MMP), Western blot, and apoptotic analysis, which indicated that MNLs down-regulated the cell viability and IC50 in a concentration-dependent manner and induced a significant change in JC-1 fluorescence from red to green.

Results:The above observations resulted in increased Bax and Caspase-3 levels, coupled with a substantial decrease in Bcl-2 and apoptotic promotion at the advanced stage compared with MA.

Conclusion:Based on these results, MNLs may serve as a more effective and promising therapeutic option for ESCC.

About the authors

Kai Zhao

Department of Gastroenterology, Jintan Hospital Affiliated to Jiangsu University

Author for correspondence.
Email: info@benthamscience.net

Yun Cai

Department of Gastroenterology, Jintan Hospital Affiliated to Jiangsu University

Email: info@benthamscience.net

Faisal Raza

School of Pharmacy, Shanghai Jiao Tong University

Email: info@benthamscience.net

Hajra Zafar

School of Pharmacy, Shanghai Jiao Tong University

Email: info@benthamscience.net

Liang Pan

Department of Gastroenterology, Jintan Hospital Affiliated to Jiangsu University

Email: info@benthamscience.net

Xifeng Zheng

Department of Gastroenterology, Jintan Hospital Affiliated to Jiangsu University

Email: info@benthamscience.net

Wenjie Xu

Department of Gastroenterology, Jintan Hospital Affiliated to Jiangsu University

Email: info@benthamscience.net

Ran Li

School of Pharmacy, Jiangsu University

Email: info@benthamscience.net

Feng Shi

School of Pharmacy, Jiangsu University

Author for correspondence.
Email: info@benthamscience.net

Yongbin Ma

Department of Central Laboratory, Jintan Hospital Affiliated to Jiangsu University

Author for correspondence.
Email: info@benthamscience.net

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