Protective Effect and Related Mechanism of Modified Danggui Buxue Decoction on Retinal Oxidative Damage in Mice based on Network Pharmacology


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Abstract

Introduction:Age-related macular degeneration (AMD) is one of the common diseases that cause vision loss in the elderly, and oxidative stress has been considered a major pathogenic factor for AMD. Modified Danggui Buxue Decoction (RRP) has a good therapeutic effect on non-proliferatic diabetic retinopathy and can improve the clinical symptoms of patients. Aim: This study aimed to predict and verify the protective effect and mechanism of RRP on retinal oxidative damage in mice based on network pharmacology and animal experiments.

Methods:The key ingredients and core targets of RRP protecting retinal oxidative damage were obtained by Network pharmacology analysis. A mouse retinal oxidative damage model induced by tail vein injection of 1% NaIO3 solution (25 mg/kg) was treated with RRP for 4 weeks and used to verify the pharmacodynamics and related mechanism.

Aim:This study aimed to predict and verify the protective effect and mechanism of RRP on retinal oxidative damage in mice based on network pharmacology and animal experiments

Results:A total of 15 key active components included in RRP interacted with 57 core targets related to retinal oxidative damage (such as AKT1, NFE2L2, HMOX1), mainly involved in the AGE-RAGE signaling pathway in diabetic complications, PI3K-AKT signaling pathway and so on. Further studies in vivo found that RRP improved the retinal oxidative damage, increased the content of SOD and GSH, decreased the content of MDA in mouse serum, promoted the expression of p-PI3K, p-AKT, Nrf2, HO-1 and NQO1 proteins in the mouse retina, and inhibited the expression of Nrf2 in the cytoplasm.

Conclusion:This study revealed that RRP had a protective effect on oxidative damage of the retina in mice, and might exert anti-oxidative effect by activating the PI3K/Akt/Nrf2 signal pathway. This study provided scientific data for the further development of hospital preparations of RRP, and a good theoretical basis for the clinical application of RRP.

About the authors

Xiangka Hu

Institute of Materia Medica, Jinzhou Medical University

Email: info@benthamscience.net

Feifei Liu

Department of Anesthesiology, The First Affiliated Hospital of Jinzhou Medical University

Email: info@benthamscience.net

He Yang

College of Basic Medicine, Jinzhou Medical University

Email: info@benthamscience.net

Mushuang Qi

College of Basic Medicine, Jinzhou Medical University

Email: info@benthamscience.net

Ying Ren

College of Basic Medicine, Jinzhou Medical University

Email: info@benthamscience.net

Wanjun Zhu

College of Basic Medicine, Jinzhou Medical University

Email: info@benthamscience.net

Chunmei Dai

Institute of Materia Medica, Jinzhou Medical University

Author for correspondence.
Email: info@benthamscience.net

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