Effect of Raltitrexed on ECA109 Cellular Radiosensitivity and its Mechanism in Esophageal Cancer


Cite item

Full Text

Abstract

Background:To investigate the effect of raltitrexed + X-ray irradiation on esophageal cancer ECA109 cells and analyze the potential action mechanism

Methods:The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to analyze the inhibitory effect of raltitrexed on cell proliferation. The effect of raltitrexed on radiosensitivity was studied through a clone-forming experiment. The scratch assay and invasion test were performed to understand the cell migration and invasion abilities. The apoptosis rate change was measured using a flow cytometer, and Western Blotting was used to determine the expression of B cell lymphoma-2 (Bcl-2) and Bcl2-associated X protein (Bax) in each group.

Results:Raltitrexed significantly inhibited ECA109 proliferation in a time-dose-dependent manner; there were significant differences among different concentrations and times of action. The results of the clone-forming experiment showed a sensitization enhancement ratio of 1.65, and this demonstrated a radiosensitization effect. After the combination of raltitrexed with X-ray, the cell migration distance was shortened, and the number of cells penetrating the membrane was reduced.

Conclusion:Raltitrexed can inhibit the growth of esophageal cancer ECA109 cells and has a radiosensitization effect.

About the authors

Li-Ben Xu

Department of Radiotherapy, First Affiliated Hospital of Soochow University

Email: info@benthamscience.net

Chao-Yang Wu

Department of Radiotherapy, Jiangsu University Affiliated People's Hospital

Email: info@benthamscience.net

Yan Wang

Department of Radiotherapy, Zhenjiang First People's Hospital

Email: info@benthamscience.net

Ju-Ying Zhou

Department of Radiotherapy, First Affiliated Hospital of Soochow University

Author for correspondence.
Email: info@benthamscience.net

References

  1. Yano T. Paradox of age-specific incidence rates of second primary cancer in individuals with esophageal cancer. J Gastroenterol 2020; 55(6): 664-5. doi: 10.1007/s00535-020-01687-7 PMID: 32285194
  2. Gong J, Wang MM, Zhang XH, et al. Efficacy and safety of huachansu capsules for the treatment of esophageal cancer: A systematic review and meta-analysis. World J Tradit Chin Med 2023; 9(3): 270-7. doi: 10.4103/2311-8571.355009
  3. Kelly RJ. Emerging multimodality approaches to treat localized esophageal cancer. J Natl Compr Canc Netw 2019; 17(8): 1009-14. doi: 10.6004/jnccn.2019.7337 PMID: 31390584
  4. Chen JQ, Lin Y, Su TF, et al. Clinical study of non-operative staging of esophageal squamous cell carcinoma underwent intensity- modulated radiotherapy combined with chemotherapy. Chin J Radiat Oncol 2017; 26: 155-9.
  5. Tang JC, Zhao J, Long F, et al. Efficacy of shikonin against esophageal cancer cells and its possible mechanisms in vitro and in vivo. J Cancer 2018; 9(1): 32-40. doi: 10.7150/jca.21224 PMID: 29290767
  6. Long L, Pang XX, Lei F, et al. SLC52A3 expression is activated by NF-κB p65/Rel-B and serves as a prognostic biomarker in esophageal cancer. Cell Mol Life Sci 2018; 75(14): 2643-61. doi: 10.1007/s00018-018-2757-4 PMID: 29428966
  7. Sun HH, He LM, Zhang JD, Shi DM. Effects of raltitrexed combined with nedaplatin on the expression of proline rich 11 and squamous cell carcinoma antigen in patients with esophageal squamous cell carcinoma. Oncol Prog 2020; 18: 1345-8.
  8. Wang S, Li X, Qian TK. Synergistic effect of Raltetrexed combined with X-ray irradiation on human laryngeal carcinoma cells Hep-2. J Clin Med 2017; 24: 879-83.
  9. Stausbøl-Grøn B, Nielsen OS, Bentzen SM, Overgaard J. Selective assessment of in vitro radiosensitivity of tumour cells and fibroblasts from single tumour biopsies using immunocytochemical identification of colonies in the soft agar clonogenic assay. Radiother Oncol 1995; 37(2): 87-99. doi: 10.1016/0167-8140(95)98589-D PMID: 8747932
  10. Franken NAP, Rodermond HM, Stap J, Haveman J, van Bree C. Clonogenic assay of cells in vitro. Nat Protoc 2006; 1(5): 2315-9. doi: 10.1038/nprot.2006.339 PMID: 17406473
  11. Willers H, Dahm-Daphi J, Powell SN. Repair of radiation damage to DNA. Br J Cancer 2004; 90(7): 1297-301. doi: 10.1038/sj.bjc.6601729 PMID: 15054444
  12. Wu G, Chen G, Zhou J, Zhu H, Chu J, Zhang F. Liriodenine enhances radiosensitivity in esophageal cancer ECA-109 cells by inducing apoptosis and G2/M arrest. Oncol Lett 2018; 16(4): 5020-6. doi: 10.3892/ol.2018.9253 PMID: 30250568
  13. Desvoyes B, Gutierrez C. Roles of plant retinoblastoma protein: Cell cycle and beyond. EMBO J 2020; 39(19): e105802. doi: 10.15252/embj.2020105802 PMID: 32865261
  14. Li J, Yang CX, Mei ZJ, et al. Involvement of cdc25c in cell cycle alteration of a radioresistant lung cancer cell line established with fractionated ionizing radiation. Asian Pac J Cancer Prev 2013; 14(10): 5725-30. doi: 10.7314/APJCP.2013.14.10.5725 PMID: 24289569
  15. Wu X, Roth JA, Zhao H, et al. Cell cycle checkpoints, DNA damage/repair, and lung cancer risk. Cancer Res 2005; 65(1): 349-57. doi: 10.1158/0008-5472.349.65.1 PMID: 15665313
  16. Solier S, Zhang YW, Ballestrero A, Pommier Y, Zoppoli G. DNA damage response pathways and cell cycle checkpoints in colorectal cancer: Current concepts and future perspectives for targeted treatment. Curr Cancer Drug Targets 2012; 12(4): 356-71. doi: 10.2174/156800912800190901 PMID: 22385513
  17. Chen Z, Wu Y, Meng Q, Xia Z. Elevated microRNA-25 inhibits cell apoptosis in lung cancer by targeting RGS3. In Vitro Cell Dev Biol Anim 2016; 52(1): 62-7. doi: 10.1007/s11626-015-9947-2 PMID: 26416661
  18. Bunn PA Jr, Gaspar LE, Weyant MJ, Mitchell JD. Neoadjuvant combination chemotherapy for unresectable stage III non–small cell lung cancer. Cancer 2016; 122(5): 674-5. doi: 10.1002/cncr.29797 PMID: 26700372
  19. Hogg SJ, Newbold A, Vervoort SJ, et al. BET inhibition induces apoptosis in aggressive b-cell lymphoma via epigenetic regulation of BCL-2 family members. Mol Cancer Ther 2016; 15(9): 2030-41. doi: 10.1158/1535-7163.MCT-15-0924 PMID: 27406984
  20. Hahnvajanawong C, Wattanawongdon W, Chomvarin C, et al. Synergistic effects of isomorellin and forbesione with doxorubicin on apoptosis induction in human cholangiocarcinoma cell lines. Cancer Cell Int 2014; 14(1): 68. doi: 10.1186/1475-2867-14-68 PMID: 25866479
  21. Karimi F, Karimi-Maleh H, Rouhi J, et al. Revolutionizing cancer monitoring with carbon-based electrochemical biosensors. Environ Res 2023; 239(Pt 2): 117368. doi: 10.1016/j.envres.2023.117368 PMID: 37827366
  22. Tao C, Rouhi J. A biosensor based on graphene oxide nanocomposite for determination of carcinoembryonic antigen in colorectal cancer biomarker. Environ Res 2023; 238(Pt 1): 117113. doi: 10.1016/j.envres.2023.117113 PMID: 37696325

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2024 Bentham Science Publishers