The effect of space flight factors on the interaction of Escherichia coli with bacteriophage T7

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For the first time, the interaction between bacteria and bacteriophage was studied under space conditions. The model system of E. coli and bacteriophage T7 was used. The results of the interaction depended on the duration of exposure of the system to space flight factors. During the first 2 days of microgravity exposure the virus replication rate in Space was higher than on Earth. The bacteria then have adapted to space conditions and acquired resistance to the bacteriophage, which persisted for 2 days after return to Earth. Over the next three days, the sensitivity of the E. coli to the T7 bacteriophage returned to its original level.

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Sobre autores

N. Sykilinda

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences

Autor responsável pela correspondência
Email: sykilinda@mail.ru
Rússia, Moscow, 117997

A. Lukianova

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences

Email: sykilinda@mail.ru
Rússia, Moscow, 117997

V. Lavrikova

JSC “BIOHIMMASH”

Email: sykilinda@mail.ru
Rússia, Moscow, 127299

I. Kutnik

Yu.A. Gagarin Research and Test Cosmonaut Training Center

Email: sykilinda@mail.ru
Rússia, Star City, Moscow region, 141160

N. Panin

Lomonosov Moscow State University

Email: sykilinda@mail.ru

Research Belozersky Institute of Physical and Chemical Biology

Rússia, Moscow, 119991

N. Staritsyn

JSC “BIOHIMMASH”

Email: sykilinda@mail.ru
Rússia, Moscow, 127299

K. Miroshnikov

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences

Email: sykilinda@mail.ru
Rússia, Moscow, 117997

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2. Fig. 1. Laying out the “Microvir” system in an open position with installed cassettes (a), external view of the “Microvir” cassette (b).

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3. Fig. 2. Photographs of the NA “Microvir” cassettes immediately after moving the contents of the upper wells to the lower ones in CE (a) and NE (b). Photographs of the NA “Microvir” cassettes after completion of cell lysis in CE (c) and NE (d).

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4. Fig. 3. Sensitivity of control E. coli cells from CE to bacteriophage T7 after the return of the Microvir NA from the ISS (a): 1 – 1 day, 2 – 2 days, 3 – 3 days (experiment of the second day), 4 – 3 days (experiment of the first day), 5 – 5 days (experiment of the second day), 6 – 5 days (experiment of the first day). Sensitivity of control E. coli cells from NE to bacteriophage T7 after the completion of the experiment (b): 1 – 1 day, 2 – 1 day, 3 – 3 days, 4 – 5 days.

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