A New Method for Express Detection of Antibiotic Resistance

S. N. Pleskova; Плескова С. Н.; E. V. Lazarenko; Лазаренко Е. В.; I. S. Sudakova; Судакова И. С.; R. N. Kriukov; Крюков Р. Н; N. A. Bezrukov; Безруков Н. А.

doi:10.31857/S0555109923010075

A New Method for Express Detection of Antibiotic Resistance

作者: Pleskova S.N.¹^,2, Lazarenko E.V.¹^,2, Sudakova I.S.², Kriukov R.N.¹, Bezrukov N.A.¹^,2
隶属关系:
1. N.I. Lobachevsky Nizhny Novgorod State University, Ministry of Science and Higher Education
2. R.E. Alekseev Nizhny Novgorod State Technical University, Ministry of Science and Higher Education
期: 卷 59, 编号 1 (2023)
页面: 74-80
栏目: Articles
URL: https://vestnikugrasu.org/0555-1099/article/view/674645
DOI: https://doi.org/10.31857/S0555109923010075
EDN: https://elibrary.ru/CVOIPK
ID: 674645

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详细

The oscillation mode of an atomic force microscope (AFM) was used to create a highly sensitive real-time detection system for antibiotic resistance. This mode allows to evaluate the sensitivity or resistance of Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria to an antibiotic in 15–30 minutes. The analytical signal (changes in the amplitude-frequency characteristics of the cantilever) is based on the metabolic activity of bacteria. Bacteria was adding on the cantilever and was causing it to oscillate with high amplitude. If the bacteria are sensitive to the antibiotic, the amplitude drops statistically significant within 15–30 minutes, if the bacteria are resistant, then the amplitude either does not change or increases. The obtained results were comparable with the disk diffusion method.

关键词

antibiotic resistance, atomic force microscopy, oscillation mode, disk diffusion method, Escherichia coli, Staphylococcus aureus

参考

Crofts T.S., Gasparrini A.J., Dantas G. // Nat. Rev. Microbiol. 2017. V. 15. P. 422–434.
Davies J., Davies D. // Microbiol. Mol. 2010. V. 74. № 3. P. 417–433.
Liu B., Pop M. // Nucleic Acids Res. 2009. V. 37. № 1. P. 443–447.
Nathwani D., Varghese D., Stephens J., Ansari W., Martin S., Charbonneau C. // Antimicrob. Resist. Infect. Control. 2019. V. 8. № 35. https://doi.org/10.1186/s13756-019-0471-0
CLSI, Performance Standards for Antimicrobial Disk Susceptibility Tests, Approved Standard / Ed. 7, CLSI document M02-A11. Clinical and Laboratory Standards Institute, 2012.
CLSI, Method for Antifungal Disk Diffusion Susceptibility Testing of Yeasts, Approved Guideline. / Ed. 2, CLSI document M44-A. Clinical and Laboratory Standards Institute, 2004.
Balouiri M., Sadiki M., Ibnsouda S.K. // J. Pharm. Anal. 2016. V. 6. № 2. P. 71–79.
Kasas S., Ruggeri F.S., Benadiba C., Maillard C., Stupar P., Tournu H. et al. // Proc. Natl. Acad. Sci. 2015. V. 112. № 2. P. 378–381.
Venturelli L., Kohler A.C., Stupar P., Villalba M.I., Kalauzi A., Radotic K. et al. // J. Mol. Recognit. 2020. V. 33. № 12. P. 1–14.
Pleskova S.N., Fomichev O.I., Kriukov R.N, Sudakova I.S. // Biophysics. 2021. V. 66. № 6. P. 950–955.
Villalba M.I., Stupar P., Chomicki W., Bertacchi M., Dietler G., Arnal L. et al. // Small. 2018. V. 14. № 4. 1702671. https://doi.org/10.1002/smll.201702671
Kasas S., Malovichko A., Villalba M.I., Vela M.E., Yantorno O., Willaert R.G. // Antibiotics. 2021. V. 10. № 3. 287. https://doi.org/10.3390/antibiotics10030287
Pal S., Sayana A., Joshi A., Juyal D. // J. Family Med. Prim. Care. 2019. V. 8. № 11. P. 3600–3606.
Lee D.S., Lee S.J., Choe H.S. // Biomed. Res. Int. 2018. V. 26. 7656752. https://doi.org/10.1155/2018/7656752
Willaert R.G., Vanden Boer P., Malovichko A., Alioscha-Perez M., Radotic K., Bartolic D. et al. // Sci. Adv. 2020. V. 6. № 26. eaba3139. https://doi.org/10.1126/sciadv.aba3139
Munita J.M., Cesar A. // Microbiol. Spectr. 2016. V. 4. № 2. 1128. https://doi.org/10.1128/microbiolspec.VMBF-0016-2015