A New Method for Express Detection of Antibiotic Resistance

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The oscillation mode of an atomic force microscope (AFM) was used to create a highly sensitive real-time detection system for antibiotic resistance. This mode allows to evaluate the sensitivity or resistance of Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria to an antibiotic in 15–30 minutes. The analytical signal (changes in the amplitude-frequency characteristics of the cantilever) is based on the metabolic activity of bacteria. Bacteria was adding on the cantilever and was causing it to oscillate with high amplitude. If the bacteria are sensitive to the antibiotic, the amplitude drops statistically significant within 15–30 minutes, if the bacteria are resistant, then the amplitude either does not change or increases. The obtained results were comparable with the disk diffusion method.

作者简介

S. Pleskova

N.I. Lobachevsky Nizhny Novgorod State University,
Ministry of Science and Higher Education; R.E. Alekseev Nizhny Novgorod State Technical University,
Ministry of Science and Higher Education

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Email: pleskova@mail.ru
Russia, 603950, Nizhny Novgorod; Russia, 603115, Nizhny Novgorod

E. Lazarenko

N.I. Lobachevsky Nizhny Novgorod State University,
Ministry of Science and Higher Education; R.E. Alekseev Nizhny Novgorod State Technical University,
Ministry of Science and Higher Education

Email: pleskova@mail.ru
Russia, 603950, Nizhny Novgorod; Russia, 603115, Nizhny Novgorod

I. Sudakova

R.E. Alekseev Nizhny Novgorod State Technical University,
Ministry of Science and Higher Education

Email: pleskova@mail.ru
Russia, 603115, Nizhny Novgorod

R. Kriukov

N.I. Lobachevsky Nizhny Novgorod State University,
Ministry of Science and Higher Education

Email: pleskova@mail.ru
Russia, 603950, Nizhny Novgorod

N. Bezrukov

N.I. Lobachevsky Nizhny Novgorod State University,
Ministry of Science and Higher Education; R.E. Alekseev Nizhny Novgorod State Technical University,
Ministry of Science and Higher Education

Email: pleskova@mail.ru
Russia, 603950, Nizhny Novgorod; Russia, 603115, Nizhny Novgorod

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版权所有 © С.Н. Плескова, Е.В. Лазаренко, И.С. Судакова, Р.Н Крюков, Н.А. Безруков, 2023