Development of Kinetic Microplate Immunoenzyme Determination of Dibutyl Phthalate

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Abstract

Phthalic acid esters are integral components of modern plastic products and packaging materials, which causes significant contamination of food products and the environment, leading to the need for simple productive monitoring methods. The article presents a rapid enzyme-linked immunosorbent assay (ELISA) for the determination of dibutyl phthalate (DBP) in fruit juices, based on the competitive interaction between free and bound antigen for the binding sites of specific antibodies. The analytical characteristics of the method were studied in various kinetic regimes of the competition stage. Optimal conditions have been established to ensure the minimum detection limit and high measurement accuracy. The duration of the competitive stage of ELISA was chosen 30 min; the range of determined concentrations of DBP was from 0.37 to 68.34 ng/mL with a detection limit of 0.08 ng/mL. The efficiency of the proposed ELISA for testing fruit juices was shown for the chosen DBP extraction mode.

About the authors

A. N. Berlina

A.N. Bach Institute of Biochemistry. Research Center of Biotechnology
of the Russian Academy of Sciences

Email: dzantiev@inbi.ras.ru
Russia, 119071, Moscow

K. V. Serebrennikova

A.N. Bach Institute of Biochemistry. Research Center of Biotechnology
of the Russian Academy of Sciences

Email: dzantiev@inbi.ras.ru
Russia, 119071, Moscow

N. S. Komova

A.N. Bach Institute of Biochemistry. Research Center of Biotechnology
of the Russian Academy of Sciences

Email: dzantiev@inbi.ras.ru
Russia, 119071, Moscow

A. V. Zherdev

A.N. Bach Institute of Biochemistry. Research Center of Biotechnology
of the Russian Academy of Sciences

Email: dzantiev@inbi.ras.ru
Russia, 119071, Moscow

B. B. Dzantiev

A.N. Bach Institute of Biochemistry. Research Center of Biotechnology
of the Russian Academy of Sciences

Author for correspondence.
Email: dzantiev@inbi.ras.ru
Russia, 119071, Moscow

References

  1. Chen Y., He Q., Shen D., Jiang Zh., Eremin S.A., Zhao S. // Food Control. 2019. V. 105. P. 38–44. https://doi.org/10.1016/j.foodcont.2018.11.052
  2. Huang Zh., Tu Ch., Liu H., Wang L., Zhu Z., Watanabe I. // J. Chromatogr. A. 2020. V. 1619. Article 460953. https://doi.org/10.1016/j.chroma.2020.460953
  3. Yan Y., Lu Y., Wang B., Gao Y., Zhao L., Liang H., Wu D. // ACS Appl. Mater. Interfaces. 2018. V. 10. № 31. P. 26539–26545. https://doi.org/10.1021/acsami.8b08934
  4. Tang M., Wu Y., Deng D., Wei J., Zhang J., Yang D., Li G. // Sens. Actuators B Chem. 2018. V. 258. P. 304–312. https://doi.org/10.1016/j.snb.2017.11.120
  5. Giuliani A., Zuccarini M., Cichelli A., Khan H., Reale M. // Int. J. Environ. Health Res. 2020. V. 17. № 16. Article 5655. https://doi.org/10.3390/ijerph17165655
  6. Zhou Y., Li J., Zhang L., Ge Z., Wang X., Hu X., Xu T., Li P. // Anal. Bioanal. Chem. 2019. V. 411. № 22. P. 5691–5701. https://doi.org/10.1007/s00216-019-01947-3
  7. Luo H., Liu C., He D., Sun J., Li J., Pan X. // Sci. Total Environ. 2022. V. 849. Article 157951. https://doi.org/10.1016/j.scitotenv.2022.157951
  8. Guo W., Li J., Luo M., Mao Y., Yu X., Elskens M., Baeyens W., Gao Y. // Water Res. 2022. V. 214. Article 118189. https://doi.org/10.1016/j.watres.2022.118189
  9. Zhu N., Zou Y., Huang M., Dong S., Wu X., Liang G., Han Z., Zhang Z. // Talanta. 2018. V. 186. P. 104–109. https://doi.org/10.1016/j.talanta.2018.04.023
  10. Baranovskaya V.S., Berlina A.N., Eremin S.A. // J. Anal. Chem. 2022. V. 77. № 4. P. 466–472. https://doi.org/10.1134/S1061934822040037
  11. Montuori P., Jover E., Morgantini M., Bayona J.M., Triassi M. // Food Addit. Contam. – Chem. Anal. Control Expo. Risk Assess. 2008. V. 25. № 4. P. 511–518. https://doi.org/10.1080/02652030701551800
  12. Adeniyi A.A., Okedeyi O.O., Yusuf K.A. // Environ. Monit. Assess. 2011. V. 172. № 1. P. 561–569. https://doi.org/10.1007/s10661-010-1354-2
  13. Luís C., Algarra M., Câmara J.S., Perestrelo R. // Toxics. 2021. V. 9. № 7. Article 157. https://doi.org/10.3390/toxics9070157
  14. Otero P., Saha S.K., Moane S., Barron J., Clancy G., Murray P. // J. Chromatogr. B: Anal. Technol. Biomed. Life Sci. 2015. V. 997. P. 229–235. https://doi.org/10.1016/j.jchromb.2015.05.036
  15. Barciela-Alonso M.C., Otero-Lavandeira N., Bermejo-Barrera P. // Microchem. J. 2017. V. 132. P. 233–237. https://doi.org/10.1016/j.microc.2017.02.007
  16. Sakaki J.R., Melough M.M., Provatas A.A., Perkins Ch., Chun O.K. // Toxicol. Rep. 2020. V. 7. P. 1020–1024. https://doi.org/10.1016/j.toxrep.2020.08.015
  17. Wang X., Chen Ch., Chen Y., Kong F., Xu Zh. // Food Agric. Immunol. 2020. V. 31. № 1. P. 811–836. https://doi.org/10.1080/09540105.2020.1774746
  18. Liu S., Cheng R., Chen Y., Shi H., Zhao G. // Sens. Actuators B Chem. 2018. V. 254. P. 1157–1164. https://doi.org/10.1016/j.snb.2017.08.003
  19. Zhang Z., Zeng K., Liu J. // Trends Anal. Chem. 2017. V. 87. P. 49–57. https://doi.org/10.1016/j.trac.2016.12.002
  20. Li J., Jin H., Wei M., Ren W., Wang J., Zhang Y., Wu L., He B. // Sens. Actuators B Chem. 2021. V. 331. Article 129401. https://doi.org/10.1016/j.snb.2020.129401
  21. Berlina A.N., Ragozina M.Y., Komova N.S., Serebrennikova K.V., Zherdev A.V., Dzantiev B.B. // Biosensors. 2022. V. 12. № 11. Article 1002. https://doi.org/10.3390/bios12111002
  22. Xu F., Ren K., Yang Y.-Z., Guo J.-P., Ma G.-P., Liu Y.-M., Lu Y.-Q., Li X.-B. // J. Integr. Agric. 2015. V. 14. № 11. P. 2282–2295. https://doi.org/10.1016/S2095-3119(15)61121-2
  23. Sanchis A., Salvador J.P., Marco M.P. // Trends Anal. Chem. 2018. V. 106. P. 1–10. https://doi.org/10.1016/j.trac.2018.06.015
  24. Sun R., Zhuang H. // Food Anal. Methods. 2015. V. 8. № 8. P. 1990–1999. https://doi.org/10.1007/s12161-014-0085-3
  25. Jaria G., Calisto V., Otero M., Esteves V.I. // Anal. Bioanal. Chem. 2020. V. 412. № 17. P. 3983–4008. https://doi.org/10.1007/s00216-020-02509-8
  26. Dou L., Zhang Y., Bai Y., Li Y., Liu M., Shao Sh., Li Q., Yu W., Shen J., Wang Zh. // J. Agric. Food Chem. 2022. V. 70. № 4. P. 976–991. https://doi.org/10.1021/acs.jafc.1c06750
  27. Huebner M., Weber E., Niessner R., Boujday S., Knopp D. // Anal. Bioanal. Chem. 2015. V. 407. № 29. P. 8873–8882. https://doi.org/10.1007/s00216-015-9048-9
  28. Chen Y., Li J., Lu P., Hu D., Xue W., Ding X. // Food Agric. Immunol. 2017. V. 28. № 5. P. 904–915. https://doi.org/10.1080/09540105.2017.1320356
  29. Liu Z., Zhang Z., Zhu G., Sun J., Zou B., Li M., Wang J. // Sci. Total Environ. 2016. V. 551–552. P. 484–488. https://doi.org/10.1016/j.scitotenv.2016.02.017
  30. Xiong Y., Leng Y., Li X., Huang X., Xiong Y. // TrAC Trends Analyt. Chem. 2020. V. 126. Article 115861. https://doi.org/10.1016/j.trac.2020.115861
  31. Zhang Zh., Zhu N., Zou Y., Wu X., Qu G., Shi J. // Talanta. 2018. V. 179. P. 64–69. https://doi.org/10.1016/j.talanta.2017.10.051
  32. Zhou L., Lei Y., Zhang D., Ahmed S., Chen S. // Sci. Total Environ. 2016. V. 541. P. 570–578. https://doi.org/10.1016/j.scitotenv.2015.09.110
  33. Yanagisawa N., Dutta D. // Biosensors. 2011. V. 1. № 2. P. 58–69. https://doi.org/10.3390/bios1020058
  34. Urusov A.E., Zherdev A.V., Petrakova A.V., Sadykhov E.G., Koroleva O.V., Dzantiev B.B. // Toxins. 2015. V. 7. №. 2. P. 238–254. https://doi.org/10.3390/toxins7020238
  35. Sotnikov D.V., Zherdev A.V., Zvereva E.A., Eremin S.A., Dzantiev B.B. // Appl. Sci. 2021. V. 11. № 14. Article 6581. https://doi.org/10.3390/app11146581
  36. Wang Y., He C.H., Zheng H., Zhang H.B. // Int/ J. Mol. Sci. 2012. V. 13. № 1. P. 84–96. https://doi.org/10.3390/ijms13010084
  37. Campanella B., Palleschi V., Legnaioli S. // ChemTexts. 2021. V. 7. № 1. P. 1–21. https://doi.org/10.1007/s40828-020-00129-4
  38. Kong J., Yu S. // Acta Biochim. Biophys. Sin. 2007. V. 39. № 8. P. 549–559. https://doi.org/10.1111/j.1745-7270.2007.00320.x
  39. Ramesh S., Yin T.S., Liew C.-W. // Ionics. 2011. V. 17. № 8. P. 705–713. https://doi.org/10.1007/s11581-011-0568-9
  40. Rajamanikyam M., Vadlapudi V., Parvathaneni S.P., Koude D., Sripadi P., Misra S., Amanchy R., Upadhyayula S.M. // EXCLI J. 2017. V. 16. P. 375–387. https://doi.org/10.17179/excli2017-145
  41. Ye X., Wang P., Wu Y., Zhou Y., Sheng Y., Lao K. // Environ. Sci. Pollut. Res. 2020. V. 27. № 33. P. 42082–42091. https://doi.org/10.1007/s11356-020-10136-0
  42. Xu Zh., Xiong X., Zhao Y., Xiang W., Wu Ch. // J. Hazard. Mater. 2020. V. 384. Article 121282. https://doi.org/10.1016/j.jhazmat.2019.121282
  43. Rastkari N., Jeddi M.Z., Yunesian M., Ahmadkhaniha R. // J. Environ. Health Sci. Eng. 2018. V. 16. № 1. P. 27–33. https://doi.org/10.1007/s40201-018-0292-8
  44. Zhu F., Zhang H., Qiu M., Wu N., Zeng K., Du D. // Sci. Total Environ. 2019. V. 695. Article 133793. https://doi.org/10.1016/j.scitotenv.2019.133793
  45. Xiong D., Zhu N., Zhu F., Yakubu S., Lv J., Liu J., Zhang Z. // J. Hazard. Mater. 2022. V. 425. Article 127991. https://doi.org/10.1016/j.jhazmat.2021.127991
  46. Kuang H., Liu L., Xu L., Ma W., Guo L., Wang L., Xu C. // Sensors. 2013. V. 13. № 7. P. 8331–8339. https://doi.org/10.3390/s130708331
  47. Wei C., Ding S., You H., Zhang Y., Wang Y., Yang X., Yuan J. // PLoS One. 2011. V. 6. № 12. Article e29196. https://doi.org/10.1371/journal.pone.0029196

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