Development of Microplate Immunoenzyme Determination of Nonylphenol with Magnetic Sample Concentration

Мұқаба

Дәйексөз келтіру

Толық мәтін

Ашық рұқсат Ашық рұқсат
Рұқсат жабық Рұқсат берілді
Рұқсат жабық Тек жазылушылар үшін

Аннотация

Nonylphenol is an aromatic organic compound that has an estrogen-like effect and has a negative effect on the human endocrine system. A method has been developed for the competitive determination of nonylphenol using magnetic particles, rabbit antiserum, nonylphenol conjugate with soybean trypsin inhibitor (STI) and biotin. The principle of the analysis is the formation of immune complexes on the surface of magnetite particles due to covalent immobilization of protein G through the oriented immobilization of polyclonal antibodies from rabbit serum during a competitive reaction between the free analyte (nonylphenol) and the bound one (as part of the nonylphenol-STI-biotin conjugate) for the binding sites of specific antibodies. The detection of formed immune complexes is proposed to be carried out using a streptavidin-polyperoxidase conjugate, which makes it possible to achieve a nine-fold gain in the level of the analytical signal. The developed ELISA using magnetite particles allows us to achieve a detection limit of nonylphenol at the level of 3.8 ng/ml, which is 14.5 times lower in comparison with the classical competitive ELISA (55 ng/ml). Based on the results of the experimental work, the optimized volume of the test sample was 500 μl, which makes it possible to concentrate low-contaminated samples by 17 times.

Толық мәтін

Рұқсат жабық

Авторлар туралы

A. Berlina

Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences

Хат алмасуға жауапты Автор.
Email: dzantiev@inbi.ras.ru
Ресей, Moscow

L. Barshevskaya

Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences

Email: dzantiev@inbi.ras.ru
Ресей, Moscow

K. Serebrennikova

Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences

Email: dzantiev@inbi.ras.ru
Ресей, Moscow

N. Komova

Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences

Email: dzantiev@inbi.ras.ru
Ресей, Moscow

A. Zherdev

Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences

Email: dzantiev@inbi.ras.ru
Ресей, Moscow

B. Dzantiev

Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences

Email: dzantiev@inbi.ras.ru
Ресей, Moscow

Әдебиет тізімі

  1. Evans A.E.V., Mateo-Sagasta J., Qadir M., Boelee E., Ippolito A. // Curr. Opin. Environ. Sustain. 2019. V. 36. P. 20–27.
  2. Zamora-Ledezma C., Negrete-Bolagay D., Figueroa F., Zamora-Ledezma E., Ni M., Alexis F., Guerrero V.H. // Environ. Technol. Innov. 2021. V. 22. Article 101504. https://doi.org/10.1016/j.eti.2021.101504
  3. Fang W., Peng Y., Muir D., Lin J., Zhang X. // Environ. Int. 2019. V. 131. Article 104994. https://doi.org/10.1016/j.envint.2019.104994
  4. Fuller R., Landrigan P.J., Balakrishnan K., Bathan G., Bose-O’Reilly S., Brauer M. et al. // Lancet Planet. Health. 2022. V. 6. № 6. P. e535–e547.
  5. Palani G., Arputhalatha A., Kannan K., Lakkaboyana S.K., Hanafiah M.M., Kumar V., Marella R.K. // Molecules. 2021. V 26. № 9. Article 2799. https://doi.org/10.3390/molecules26092799
  6. Babuji P., Thirumalaisamy S., Duraisamy K., Periyasamy G. // Water. 2023. V. 15. № 14. Article 2532. https://doi.org/10.3390/w15142532
  7. Bhandari G., Bagheri A.R., Bhatt P., Bilal M. // Chemosphere. 2021. V. 275. Article 130013. https://doi.org/10.1016/j.chemosphere.2021.130013
  8. Gałązka A., Jankiewicz U. // Microorganisms. 2022. V. 10. № 11. Article 2236. https://doi.org/10.3390/microorganisms10112236
  9. Morin-Crini N., Lichtfouse E., Liu G., Balaram V., Ribeiro A.R. L., Lu Z. et al.. // Environ. Chem. Lett. 2022. V. 20. № 4. P. 2311–2338.
  10. Chen Y., Yang J., Yao B., Zhi D., Luo L., Zhou Y. // Environ. Pollut. 2022. V. 310. Article 119918. https://doi.org/10.1016/j.envpol.2022.119918
  11. Hong Y., Feng C., Yan Z., Wang Y., Liu D., Liao W., Bai Y. // Environ. Chem. Lett. 2020. V. 18. № 6. P. 2095–2106.
  12. Careghini A., Mastorgio A.F., Saponaro S., Sezenna E. // Environ. Sci. Pollut. Res. 2015. V. 22. № 8. P. 5711–5741.
  13. Jardak K., Drogui P., Daghrir R. // Environ. Sci. Pollut. Res. 2016. V. 23. № 4. P. 3195–3216.
  14. Lu D., Yu L., Li M., Zhai Q., Tian F., Chen W. // Chemosphere. 2021. V. 275. Article 129973. https://doi.org/10.1016/j.chemosphere.2021.129973
  15. Noorimotlagh Z., Mirzaee S.A., Martinez S.S., Rachoń D., Hoseinzadeh M., Jaafarzadeh N. // Environ Res. 2020. V. 184. Article 109263. https://doi.org/10.1016/j.envres.2020.109263
  16. Directive 2013/39/eu of the European parliament and of the council of 12 August 2013 amending Directives 2000/60/EC and 2008/105/EC as regards priority substances in the field of water policy.
  17. Shih H.-K., Shu T.-Y., Ponnusamy V. K., Jen J.-F. // Anal. Chim. Acta. 2015. V. 854. P. 70–77.
  18. Vargas-Berrones K., Díaz de León-Martínez L., Bernal-Jácome L., Rodriguez-Aguilar M., Ávila-Galarza A., Flores-Ramírez R. // Talanta. 2020. V. 209. Article 120546. https://doi.org/10.1016/j.talanta.2019.120546
  19. Aparicio I., Martín J., Santos J.L., Malvar J.L., Alonso E. // J. Chromatogr. A. 2017. V. 1500. P. 43–52.
  20. Yin H.-L., Zhou T.-N. // Chinese J. Anal. Chem. 2022. V. 50. № 8. Article 100112. https://doi.org/10.1016/j.cjac.2022.100112
  21. Céspedes R., Skryjová K., Raková M., Zeravik J., Fránek M., Lacorte S., Barceló D. // Talanta. 2006. V. 70. № 4. P. 745–751.
  22. Matsui K., Kawaji I., Utsumi Y., Ukita Y., Asano T., Takeo M., Kato D.-i., Negoro S. // J. Biosci. Bioeng. 2007. V. 104. № 4. P. 347–350.
  23. Yakovleva J.N., Lobanova A.Y., Shutaleva E.A., Kourkina M.A., Mart’ianov A.A., Zherdev A.V., Dzantiev B.B., Eremin S.A. // Anal. Bioanal. Chem. 2004. V. 378. № 3. P. 634–641.
  24. Ermolaeva T.N., Dergunova E.S., Kalmykova E.N., Eremin S.A. // J. Anal. Chem. 2006. V. 61. № 6. P. 609–613.
  25. Badea M., Nistor C., Goda Y., Fujimoto S., Dosho S., Danet A., Barceló D., Ventura F., Emnéus J. // Analyst. 2003. V. 128. № 7. P. 849–856.
  26. Mart’ianov A.A., Zherdev A.V., Eremin S.A., Dzantiev B.B. // Int. J. Env. Anal. Chem. 2004. V. 84. № 13. P. 965–978.
  27. Mart’ianov A.A., Dzantiev B.B., Zherdev A.V., Eremin S.A., Cespedes R., Petrovic M., Barcelo D. // Talanta. 2005. V. 65. № 2. P. 367–374.
  28. Berlina A.N., Komova N.S., Serebrennikova K.V., Zherdev A.V., Dzantiev B.B. // Engineering Proceedings. 2023. V. 48. № 1. Article 9. https://doi.org/10.3390/CSAC2023–14919.
  29. Berlina A.N., Ragozina M.Y., Gusev D.I., Zherdev A.V., Dzantiev B.B. // Chemosensors. 2023. V. 11. № 7. Article 393. https://doi.org/10.3390/chemosensors11070393.
  30. Kuang H., Liu L., Xu L., Ma W., Guo L., Wang L., Xu C. // Sensors. 2013. V. 13. № 7. P. 8331–8339.
  31. Kato M., Ihara Y., Nakata E., Miyazawa M., Sasaki M., Kodaira T., Nakazawa H. // Food and Agricultural Immunology. 2007. V. 18. № 3–4. P. 179–187.

Қосымша файлдар

Қосымша файлдар
Әрекет
1. JATS XML
2. Fig. 1. Scheme of ELISA with magnetic preconcentration.

Жүктеу (16KB)
3. Fig. 2. Absorption spectrum of the NF-SIT conjugate. The cuvette thickness is 1 mm, the concentration of the conjugate in 10 mM FBS is 1.2 mg/ml.

Жүктеу (2KB)
4. Fig. 3. Characterization of antiserum by ELISA: linear section of the competitive interaction curve (n = 3).

Жүктеу (1KB)
5. Fig. 4. Testing the preservation of immunochemical activity of the hapten-protein conjugate before and after biotinylation (n = 2).

Жүктеу (16KB)
6. Fig. 5. Testing of biotin-streptavidin binding in the NF-SIT-biotin preparation and selection of concentrations of the ST-HRP conjugate (A) and ST-rHRP (B) (n = 2).

Жүктеу (5KB)
7. Fig. 6. Selection of the optimal concentration of the G-IgG protein (based on the concentration of magnetic particles) (n = 3).

Жүктеу (12KB)
8. Fig. 7. Determination of the optimal concentration of the NF-SIT-biotin conjugate (n = 2). The dotted line indicates the cutoff at optical density 1.0.

Жүктеу (13KB)
9. Fig. 8. Calibration curve for the determination of NF using the developed system based on MP (n = 3).

Жүктеу (1KB)
10. Fig. 9. Dependence of the analytical signal in the developed ELISA based on MPs on the volume in which the concentration took place (n = 3).

Жүктеу (14KB)

© Russian Academy of Sciences, 2024