Phylogeny and characterization of endophytic bacteria isolated from the rhizosphere of pea seedlings (Pisum sativum L.)

Cover Page

Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription Access

Abstract

We have previously shown the ability of endophytic bacteria to move out of the pea plant seedling roots (Pisum sativum L.) into the rhizosphere. In this study, six distinct bacterial strains were isolated from the root growth medium during the cultivation of seedlings in an aqueous medium. By analyzing the nucleotide sequence of 16S rRNA genes, the taxonomic position of these strains was established, their morphological and cultural parameters were assessed, the activity of hydrolytic enzymes (pectinase, cellulase, protease) and the IAA-producing capability were examined. It has been observed that the quantity of endophytic bacteria that appears on the root surface during the growth of pea seedlings significantly surpasses the quantity present in the root tissues. It is assumed that hydrolytic enzymes such as pectinase and cellulase are involved in the release of bacteria into the external environment, causing the destruction of carbohydrate structures in plant cell walls. The metabolic parameters established in the studied strains and the significance of these endophytic bacteria for the host plant after their exit from the roots into the rhizosphere are under discussion.

Full Text

Restricted Access

About the authors

L. E. Makarova

Sibirian Institute of Plant Physiology and Biochemistry (SIPPB SB RAS)

Author for correspondence.
Email: makarova@sifibr.irk.ru
Russian Federation, Irkutsk, 664033

Yu. A. Markova

Sibirian Institute of Plant Physiology and Biochemistry (SIPPB SB RAS)

Email: makarova@sifibr.irk.ru
Russian Federation, Irkutsk, 664033

Yu. V. Zaytseva

Demidov Yaroslavl State University

Email: makarova@sifibr.irk.ru
Russian Federation, Yaroslavl ,150003

A. A. Bychkova

Demidov Yaroslavl State University

Email: makarova@sifibr.irk.ru
Russian Federation, Yaroslavl ,150003

I. V. Gorbenko

Sibirian Institute of Plant Physiology and Biochemistry (SIPPB SB RAS)

Email: makarova@sifibr.irk.ru
Russian Federation, Irkutsk, 664033

Yu. M. Konstantinov

Sibirian Institute of Plant Physiology and Biochemistry (SIPPB SB RAS); Irkutsk State University

Email: makarova@sifibr.irk.ru
Russian Federation, Irkutsk, 664033

I. A. Vasiliev

Sibirian Institute of Plant Physiology and Biochemistry (SIPPB SB RAS)

Email: makarova@sifibr.irk.ru
Russian Federation, Irkutsk, 664033

A. S. Morits

Sibirian Institute of Plant Physiology and Biochemistry (SIPPB SB RAS)

Email: makarova@sifibr.irk.ru
Russian Federation, Irkutsk, 664033

P. A. Bizikov

Sibirian Institute of Plant Physiology and Biochemistry (SIPPB SB RAS)

Email: makarova@sifibr.irk.ru
Russian Federation, Irkutsk, 664033

References

  1. Muresu R., Polone E., Sulas L., Baldan B., Tondello A., Delogu G., Cappuccinelli P. et al. // FEMS Microbiol Ecol. 2008. V. 63. P. 383–400.
  2. Гарипова С.Р. // Успехи современной биологии. 2012. Т. 132. № 11. С. 493–505.
  3. Чеботарь В.К., Мальфанова Н.В., Щербаков А.В., Ахтемова Г.А., Борисов А.Ю., Люгтенберг Б., Тихонович И.А. // Прикл. биохимия и микробиология. 2015. Т. 51. № 3. С. 283–289. https://doi.org/10.7868/S0555109915030058
  4. Васильева Е.Н., Ахтемова Г.А., Жуков В.А., Тихонович И.А. // Экологическая генетика. 2019. Т. 17. № 1. С. 19–32. https://doi.org/10.17816/ecogen17119-32
  5. Гарипова С.Р., Гарифуллина Д.В., Баймиев А.Х., Хайруллин Р.М. // Прикл. биохимия и микробиология. 2017. Т. 53. № 3. С. 299–307.
  6. Martinez-Hidalgo P., Hirsh A.M. // Phytobioms. 2017. V. 1. № 2. P. 70–82.
  7. Benito P., Alonso-Vega P., Aguado C., Luján R., Anzai Y., Hirsch A.M., Trujillo M.E. // Sc. Rep. 2017. V. 7. Art. 11051. https://doi.org/10.1038/s41598-017-11428-1
  8. Dudeja S.S., Giri R., Saini R., Suneja-Madan P., Kothe E. // J. Basic Microbiol. 2012. V. 52. № 3. P. 248–260.
  9. Макарова Л.Е., Маркова Ю.А., Мориц А.С., Карепова М.С., Сидоров А.В., Соколова Н.А. // Прикл. биохимия и микробиология. 2021. Т. 57. № 4. С. 394–401. https://doi.org/10.31857/S0555109921040103
  10. Макарова Л.Е., Смирнов В.И., Клыба Л.В., Петрова И.Г., Дударева Л.В. // Прикл. биохимия и микробиология. 2012. Т. 48. № 4. С. 394–402
  11. Шафикова Т.Н., Омеличкина Ю.В., Еникеев А.Г., Бояркина С.В., Гвильдис Д.Э., Семенов А.А. // Докл. Академии наук. 2018. Т. 480. № 3. С. 381–383. https://doi.org/10.7868/S0869565218150264
  12. Quast C., Pruesse E., Yilmaz P., Gerken J., Schweer T., Yarza P. et al. // Nucl. Acids. Res. 2013. V. 41 (D1). P. D590–D596. https://doi.org/10.1093/nar/gks1219
  13. Callahan B.J., McMurdie P.J., Rosen M.J., Han A.W., Johnson A.J., Holmes S.P. // Nat. Methods. 2016. V. 13. № 7. P. 581–583. https://doi.org/10.1038/nmeth.3869
  14. Katoh K., Standley D.M. // Mol. Biol. Evol. 2013. V. 30. № 4. P. 772–780. https://doi.org/10.1093/molbev/mst010
  15. Tamura K., Stecher G., Kumar S. MEGA11: // Mol. Biol. Evol. 2021. V. 38. P. 3022–3027. https://doi.org/10.1093/molbev/msab120
  16. Saitou N., Nei M. // Mol. Biol. Evol. 1987. V. 4. P. 406–425. https://doi.org/10.1093/oxfordjournals.molbev.a040454
  17. Nei M., Kumar S. Molecular Evolution and Phylogenetics. New York: Oxford University Press, 2000. 350 p.
  18. Желдакова Р.А., Мямин В.Е. Фитопатогенные микроорганизмы. Минск: БГУ, 2005. 116 с.
  19. Чугунов В.А., Ермоленко З.М., Жиглецова С.К., Мартовецкая И.И., Миронова Р.И., Жиркова Н.А. и др. // Прикл. биохимия и микробиология. 2000. Т. 36. № 6. С. 666–671.
  20. Вешняков В.А., Хабаров Ю.Г., Камакина Н.Д. // Химия растительного сырья. 2008. № 4. С. 47–50.
  21. Чумаков М.И., Горбань В.В., Ковлер Л.Е., Соловова Г.К., Кривопалов Ю.В., Васильев А.Ю., Каменева С.В. // Микробиология. 1992. Т. 61. С. 92–102.
  22. Определитель бактерий Берджи / Ред. Дж. Хоулт, Н. Криг, П. Снит, Дж. Стейли, С. Уилльямса. М.: Мир, 1997. Т. 1. С. 421.
  23. Определитель бактерий Берджи / Ред. Дж. Хоулт, Н. Криг, П. Снит, Дж. Стейли, С. Уилльямс. М.: Мир, 1997. Т. 2. С. 799.
  24. Afzal I., IqrarI., Shinwari Z.K., Yasmin A. // Plant Growth Regul. 2017. V. 81. № 3. Р. 399–408.
  25. Иванчина Н.В., Гарипова С.Р., Хайруллин Р.М. // Агрохимия. 2018. № 4. С. 39–44.
  26. Чеботарь И.В., Лазарева А.В., Масалов Я.К., Михайлович В.М., Маянский Н.А. // Вестник РАМН. 2014. № 9–10. С. 39–50.
  27. Lin H.-R., Shu H.-Y., Lin G.-H. // Microbiol. Research. 2018. V. 216. P. 30–39. https://doi.org/10.1016/j.micres.2018.08.004
  28. He D., Wan W. // Frontiers in Microbiology. 2021. V. 12. Art 737116. https://doi.org/10.3389/fmicb.2021.737116
  29. Wan W., Qin Y., Wu H., Zuo W., He H., Tan J., Wang Y., He D. // Fronteirs in Microbiology. 2020. V. 11. Art. 752. https://doi.org/10.3389/fmicb.2020.00752
  30. Гарипова С.Р., Маркова О.В., Гарифуллина Д.В., Иванчина Н.В., Хайруллин Р.М. // Известия Уфимского научного центра РАН. 2018. № 3(1). С. 56–58.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2024 Russian Academy of Sciences