New Agronomically Valuable Strains of the Genus Streptomyces and Their Biochemical Characteristics

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Abstract

Organic farming is a global trend that increases the demand for biological preparations for use in agricultural production. The paper characterizes new actinomycete isolates from soil samples taken in various agrocenoses of the Vyatka-Kama Urals. As a result of preliminary testing (about 350 strains), strains 8Al3, N27-25 and P15-2 were isolated on the basis of antifungal activity. The cultures were identified by 16S rRNA as bacteria of the genus Streptomyces. The active substance with antifungal effect — scopafungin was identified using HPLC-MS/MS. Along with the inhibitory effect on phytopathogenic fungi, these strains produce auxins in the presence of 100 mcg/ml of L-tryptophan (17.4–20.8 mcg/ml), have cellulolytic activity and have a stimulating effect on germination and accumulation of dry biomass by wheat, clover and mustard seedlings. Possessing a complex of beneficial properties for plants, Streptomyces strains 8Al3, N27-25 and P15-2 can be used as candidates for the creation of biological preparations with fungicidal and phytostimulating effects.

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About the authors

I. G. Shirokikh

Federal Agricultural Research Center of the North-East named N.V. Rudnitsky; Vyatka State University

Author for correspondence.
Email: irgenal@mail.ru
Russian Federation, Kirov, 610007; Kirov, 610000

N. A. Bokov

Federal Agricultural Research Center of the North-East named N.V. Rudnitsky; Vyatka State University

Email: irgenal@mail.ru
Russian Federation, Kirov, 610007; Kirov, 610000

A. A. Alalykin

Vyatka State University

Email: irgenal@mail.ru
Russian Federation, Kirov, 610000

A. A. Shirokikh

Federal Agricultural Research Center of the North-East named N.V. Rudnitsky; Vyatka State University

Email: irgenal@mail.ru
Russian Federation, Kirov, 610007; Kirov, 610000

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Supplementary files

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2. Fig. 1. A heat map reflecting the differences in the size of the zones of inhibition of fungal test cultures by streptomyces strains.

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3. Fig. 2. Mass spectrum of unfragmented ions in a sample obtained from the QL of reference strain A4 with a retention time of 1.0 min.

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4. 3. Chromatogram (a) and mass spectrum (b) of the sample obtained from the QL strain 8Al3.

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5. 4. The phylogenetic position of the studied streptomyces strains and scopafungin—producing strains [N], based on the analysis of the nucleotide sequences of the 16S rRNA gene fragment.

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